Journal: Mediators of Inflammation

Article Title: Plasma Proteome Profiling of Coronary Artery Disease Patients: Downregulation of Transthyretin—An Important Event

doi: 10.1155/2020/3429541

Figure Lengend Snippet: Validation of TTR in CAD Plasma: (a) The enzyme-linked immunosorbent assay of TTR from plasma of CAD patients ( n = 200) and control ( n = 50) indicated significant low level with a 1.61-fold p < 0.0001. (b) The ROC plot indicates the individual protein abundance of each group with area under curve AUC: 0.930. The statistical significance was determined by Student's t -test, p < 0.05.

Article Snippet: The enzyme-linked immunosorbent assay (ELISA) was carried out using anti-TTR antibody (ProSci, USA).

Techniques: Biomarker Discovery, Clinical Proteomics, Enzyme-linked Immunosorbent Assay, Control, Quantitative Proteomics

Journal: International Journal of Molecular Sciences

Article Title: In Vitro and In Vivo Effects of SerpinA1 on the Modulation of Transthyretin Proteolysis

doi: 10.3390/ijms22179488

Figure Lengend Snippet: Plasmin cleaves transthyretin V30M, and its activity is inhibited by SerpinA1. Representative images of Western blotting analysis of the three independent in vitro experiments. Western blot was performed using two different antibodies targeting human TTR, mouse anti-TTR mutant (Y78F), clone AD7 ( A ), and rabbit anti-transthyretin ( B ). Both antibodies detected dimeric and monomeric TTR forms. However, only the mouse anti-TTR mutant (Y78F) clone AD7 detects TTR fragments ( A ).

Article Snippet: Mouse monoclonal antibody anti-TTR mutant (Y78F), clone AD7 was from Merck Millipore (Sigma-Merck, Darmstadt, Germany).

Techniques: Activity Assay, Western Blot, In Vitro, Mutagenesis

Journal: Transgenic Research

Article Title: Co-precipitation molecules hemopexin and transferrin may be key molecules for fibrillogenesis in TTR V30M amyloidogenesis

doi: 10.1007/s11248-017-0054-x

Figure Lengend Snippet: Histochemical and immunohistochemical analyses of amyloid deposits in the small intestine of a 7.2-hMet30 mouse kept under CV conditions. Congo red-positive deposits were present in the lamina propria of the small intestine ( a ). These deposits showed apple green birefringence under polarized light ( b ). Amyloid deposits demonstrated positive reactions with anti-human TTR antibody in our immunohistochemical analysis ( c ). Immunohistochemical analysis suggested co-precipitation of HPX in TTR amyloid deposits ( d ), and the presence of Tf ( e ). Bars = 100 μm. (Color figure online)

Article Snippet: For immunohistochemistry, the same paraffin sections were analyzed via rabbit anti-human TTR (MBL, Nagoya, Japan), anti-human hemopexin (HPX) rabbit polyclonal IgG (MBL, Nagoya, Japan), anti-mouse transferrin (Tf) rabbit polyclonal IgG (Inter-Cell Technologies, Hopewell, NJ, USA), and an avidin–biotin–peroxidase complex (ABC kit; Vector, Burlingame, CA, USA).

Techniques: Immunohistochemical staining

Journal: Transgenic Research

Article Title: Co-precipitation molecules hemopexin and transferrin may be key molecules for fibrillogenesis in TTR V30M amyloidogenesis

doi: 10.1007/s11248-017-0054-x

Figure Lengend Snippet: Schematic drawing of the enhancement of amyloidogenesis of hTTR V30M with co-precipitation of HPX or Tf. Triangles, TTR; white circles, HPX; black circles, Tf

Article Snippet: For immunohistochemistry, the same paraffin sections were analyzed via rabbit anti-human TTR (MBL, Nagoya, Japan), anti-human hemopexin (HPX) rabbit polyclonal IgG (MBL, Nagoya, Japan), anti-mouse transferrin (Tf) rabbit polyclonal IgG (Inter-Cell Technologies, Hopewell, NJ, USA), and an avidin–biotin–peroxidase complex (ABC kit; Vector, Burlingame, CA, USA).

Techniques: